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Delivery of 9‐ Cis Retinal to Photoreceptors from Bovine Serum Albumin
Author(s) -
Li Zhongzeng,
Zhuang Jianling,
Corson D. Wesley
Publication year - 1999
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1999.tb03319.x
Subject(s) - bovine serum albumin , retinoid , chemistry , retinal , serum albumin , vesicle , biochemistry , albumin , phospholipid , retina , retinoic acid , biology , membrane , gene , neuroscience
— Retinoids are labile compounds with less than micro‐molar solubility in aqueous solutions. They are normally associated with a carrier protein, interphotoreceptor retinoid binding protein, in the interstitial spaces of the retina. In the past, experimental retinoids have been delivered to isolated retinas and photoreceptors using either unprotected ethanolic suspensions or phospholipid vesicles that protect but require burdensome preparation. The objective of these experiments was to characterize the protection of retinoids and their delivery to isolated photoreceptor cells at high concentrations using fatty‐acid‐free bovine serum albumin (BSA) as a convenient vehicle and protectant. Our results confirm that BSA is a useful protectant and vehicle for delivery of retinoids to isolated retinas and photoreceptors.