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Activation of Expression of the c ‐fos Oncogene by UVA Irradiation in Cultured Human Skin Fibroblasts
Author(s) -
Bose Biplab,
Soriani Marco,
Tyrrell Rex M.
Publication year - 1999
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1999.tb03317.x
Subject(s) - enhancer , human skin , gene expression , microbiology and biotechnology , transcription factor , oncogene , transcription (linguistics) , gene , in vitro , chemistry , biology , biophysics , biochemistry , genetics , linguistics , philosophy , cell cycle
— Both broad‐spectrum and near‐monochromatic (334 nm, 365 nm and 405 nm) UVA (320–380 nm) and near‐visible radiations strongly activate accumulation of mRNA corresponding to the nuclear oncogene and transcription factor, c‐fos , in cultured human skin fibroblasts within a dose‐range encountered in the environment. The oxidizing component of UVA is clearly of central importance to the activation observed because the absence of reduced glutathione strongly enhances the response. In contrast to observations in rodent cells, we observe negligible activation of the gene in human cells after UVB (290–320 nm) radiation. The results of this study provide evidence that UVA radiation strongly activates c‐fos gene expression in human dermal fibroblasts, a phenomena that is likely to be reflected in UVA‐mediated modulation of genes containing active AP‐1‐based enhancer elements in the promoter region.