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Thermally Induced Chemiluminescence of Barley Leaves
Author(s) -
Skotnica Jirí,
Fiala Jiři,
Ilík Petr,
Dvořk Lubomír
Publication year - 1999
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1999.tb03275.x
Subject(s) - chemistry , photosystem ii , photochemistry , thermoluminescence , butylated hydroxytoluene , sodium azide , oxygen , lipid peroxidation , dcmu , luminescence , analytical chemistry (journal) , chlorophyll , photosynthesis , photosystem i , antioxidant , materials science , biochemistry , chromatography , organic chemistry , optoelectronics
— An unconventional band in the thermoluminescence glow curve of barley leaves at about +50°C was examined. In contrast to bands usually observed around +50°C, this band (designated as CL) is not related to photosynthetic electron transport in photosystem II. The appearance of the CL band (1) requires previous freezing of the sample, (2) is not influenced by light excitation and (3) depends on the presence of oxygen. In pure oxygen the glow curves for both leaves and chloroplast suspension exhibit three maxima at about +40°C, +65°C and +90°C. Based on the emission spectra of the CL band and measurements with etiolated leaves, we suppose that the majority of emission corresponding to the CL band originates from chlorophyll. A lipoxygenase inhibitor, butylated hydroxytoluene, and sodium azide decrease the intensity of the CL band. We propose that the mechanism leading to emission of the CL band involves thermally stimulated production of an active oxygen species that results in lipid peroxidation.