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The Effect of Static Magnetic Fields on the Photohemolysis of Human Erythrocytes by Ketoprofen
Author(s) -
Chignell Colin F.,
Sik Robert H.
Publication year - 1998
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1998.tb09098.x
Subject(s) - ketoprofen , chemistry , chromatography
Ultraviolet irradiation (λ > 300 nm) of the nonsteroidal anti‐inflammatory agent ketoprofen (KP, 3‐benzoyl‐α‐methylbenzoacetic acid) in aqueous solution, pH 7.4, results in heterolytic decarboxylation of the drug to give 3‐ethylbenzophenone (EtBP). Ketoprofen caused the photohemolysis of human erythrocytes probably as a result of lipid peroxidation. Application of a static magnetic field (250–1500 G) during UV (>300 nm) irradiation of KP and erythrocytes significantly decreased the time required for photohemolysis. This observation suggests that KP‐induced photohemolysis involves the initial generation of a triplet radical pair derived from the reaction of triplet state KP (or 3‐EtBP) with erythrocyte component(s) probably lipids. The magnetic field increases the concentration and/or lifetime of free radicals that escape from the radical pair so that the critical radical concentration needed to initiate membrane damage and cause cell lysis is reached sooner. Spin‐trapping studies with 2,6‐dibromo‐1‐nitrosobenzene‐4‐sulfonate confirmed that the application of an external static magnetic field increased the concentration of radicals released during the photolysis of either KP or 3‐EtBP dissolved in organized media such as sodium dodecylsulfate micelles.