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The Effects of Photodynamic Therapy on Human Neutrophil Migration Using Bacteriochlorin a
Author(s) -
Schuitmaker J. J.,
Koster B. M.,
Elferink J. G. R.
Publication year - 1998
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1998.tb05293.x
Subject(s) - photodynamic therapy , chemotaxis , superoxide , chemistry , reactive oxygen species , intracellular , microbiology and biotechnology , cell migration , receptor , biophysics , cell , biochemistry , biology , organic chemistry , enzyme
— Bacteriochlorin a photodynamic therapy (BCA‐PDT) caused inhibition of interleukin (IL)‐8‐activated neutrophil migration, at concentrations that did not induce membrane damage. Random migration and migration induced by other chemoattractants were also inhibited, indicating that the effect of BCA‐PDT was not at the level of chemoattractant‐receptor interaction but down stream. The BCA‐PDT completely blocked superoxide production of phorbol ester‐stimulated neutrophils, indicating that superoxide production by neutrophils present in the tumor before and during BCA‐PDT is not the cause of inactivation of tumor cells. Both type I and type II quenchers prevented inhibition by BCA‐PDT but only in electroporated cells. Confocal laser scanning microscopy showed that the fluorescence of BCA was located inside the cell. These results show that the effects of BCA‐PDT are intracellular and of a mixed type I/type II character and that the neutrophils present in the tumor during illumination probably do not contribute to tumor eradication by releasing reactive oxygen species.