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Chemiluminescence of Horseradish Peroxidase and Acetaldehyde Related with Gallic Acid and Hydrogen Peroxide Interaction
Author(s) -
Yoshiki Y.,
Yuan H.,
Lida T.,
Kawane M.,
Okubo K.,
Ishizawa T.,
Kawabata S.
Publication year - 1998
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1998.tb05287.x
Subject(s) - horseradish peroxidase , hydrogen peroxide , chemiluminescence , chemistry , gallic acid , acetaldehyde , peroxidase , photochemistry , peroxide , organic chemistry , enzyme , antioxidant , ethanol
Abstract— This study focuses on the fact that the chemiluminescence in the visible region is emitted from the H 2 O 2 /gallic acid/ horseradish peroxidase (HRP) and the H 2 O 2 /gallic acid acetaldehyde (MeCHO) systems. The concentration dependence of chemiluminescence intensity that led to the different response of HRP and MeCHO toward H 2 O 2 indicates that the photon emission participates with peroxidase activity including an electron transfer reaction. From our experimental results, in this study, we postulated a reaction process for chemiluminescence based on a one‐electron redox shuttle from H 2 O 2 by peroxidase. The photon intensity and spectra data from the H 2 O 2 / HRP and the H 2 O 2 /MeCHO systems with various cate‐chins were not only affected by HRP and MeCHO but also corresponded with the chemical structure of cate‐chins. The energy calculated from the spectra is 47–64 kcal/mol. These results suggested that the chemiluminescence of both systems arose from excited carbonyl compounds produced by an intermediate of the alkyl radical and the metal‐bound hydroxyl (compound II species). Hydroxyl radical inhibition, showing a notable increase from the gallic acid addition, makes the decay of the hydroxyl form of heme iron the most likely candidate for the chemiluminescence.