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Ultraviolet B Irradiation Modulates the Immune System of Fish ( Rutilus rutilus , Cyprinidae). I. Phagocytes
Author(s) -
Salo Harri M.,
Aaltonen Tuula M.,
Markkula S. Eveliina,
Jokinen E. Ilmari
Publication year - 1998
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1998.tb05223.x
Subject(s) - rutilus , respiratory burst , immune system , biology , cyprinidae , microbiology and biotechnology , fish <actinopterygii> , immunology , fishery
Roach ( Rutilus rutilus ) were irradiated with a single dose of ultraviolet B (UVB) radiation (0.4 J/cm 2 ) in order to study the effects of UVB on the nonspecific immune defense mechanisms of fish. Neutrophils and macrophages were isolated from the head kidney of fish on days 1–14 postirradiation. Both random and directed migration of neutrophils, studied by migration under agarose assay, were suppressed on day 1 after UVB irradiation. The respiratory burst of phorbol 12‐myristate 13‐acetatestimulated neutrophils and macrophages was also suppressed at days 1 and 2 after UVB irradiation. The suppression of migration and respiratory burst were restored or the responses were even enhanced later, but on the other hand spontaneous cytotoxicity of neutrophils toward 51 chromium‐labeled K562 target cells stayed suppressed throughout the 14 day follow‐up. This study indicates that UVB radiation has the potential to suppress the functioning of phagocytes and to compromise the immune system of fish.