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The Induction and Analysis of Micronuclei and Cell Killing by Ultraviolet‐B Radiation in Human Peripheral Blood Lymphocytes
Author(s) -
Keulers Rob A. C.,
Roon Angelique R.,
Roode Saskia de,
Tates Ad D.
Publication year - 1998
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1998.tb05222.x
Subject(s) - micronucleus test , clastogen , binucleated cells , microbiology and biotechnology , centromere , micronucleus , fluorescence in situ hybridization , chemistry , biology , immunology , chromosome , biochemistry , toxicity , organic chemistry , gene
ABSTRACT The DNA‐damaging potential of ultraviolet‐B (UVB) radiation was investigated by analyzing the frequency and origin of micronuclei (MN) in cytokinesis‐blocked, binucleated (BN) peripheral blood lymphocytes (PBL) and cloning efficiencies (CE) of PBL after exposure to different fluences of UVB. In total, PBL obtained from five normal donors were investigated. The PBL from all donors showed a dose‐related, linear‐quadratic increase in the frequency of MN per 1000 BN cells and in the frequency of micronucleated BN cells. In two experiments the origin of UVB‐induced MN was studied by analyzing MN for the presence or absence of centromeres by applying the MN assay in combination with a centromeric probe and fluorescence in situ hybridization. This revealed, for the first time, that UVB‐induced MN were centromere negative, indicating that UVB acted exclusively as a clastogenic agent in the tested dose range. The PBL from all donors showed a clear dose‐dependent decrease in CE, after UVB exposure. The UVB‐exposed PBL from all donors showed an inverse relationship between the induction of MN and the decrease in CE, but regression analysis revealed no correlation between the induction of MN and the decrease in cell survival. It is concluded that UVB has a clastogenic and cytotoxic effect on PBL.