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Near‐infrared Light Emission from Nanomolar‐level Singlet Molecular Oxygen Generated with an Ultra low Concentration Mixture of Hypochlorite and Hydrogen Peroxide
Author(s) -
Shiraishi Takuo,
Makiuchi Masao,
Kakinuma Katsuko,
Inaba Humio
Publication year - 1998
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1998.tb03246.x
Subject(s) - hypochlorite , singlet oxygen , chemistry , hydrogen peroxide , photochemistry , infrared , analytical chemistry (journal) , reaction rate constant , oxygen , kinetic energy , singlet state , light emission , emission intensity , kinetics , inorganic chemistry , excited state , chromatography , materials science , organic chemistry , ion , optics , physics , quantum mechanics , optoelectronics , nuclear physics
— We report the detection of a weak near‐infrared light emission originating from 8 nM singlet molecular oxygen ( 1 O 2 ) produced in a mixture of 1 m M hypochlorite (OC1 ‐ ) and 8 n M hydrogen peroxide (H 2 O 2 ). The measurements were made with a highly sensitive detection system for ultraweak light emission in the 1.0‐1.5 μm wavelength region. The emission intensity exhibited linear dependence for H 2 O 2 concentrations in the range of 8‐670 n M . The mixture containing a lower concentration (33 μ M ) of OCl ‐ pseudocontinuously emitted near‐infrared light for 5 s. The rate constant for 1 O 2 production obtained from the kinetic analysis agrees with that previously reported. Our results demonstrate the possibility of measuring very low concentrations of 1 O 2 in a OCi‐/H 2 O 2 mixture as well as 1 O 2 production in intact living systems.