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Laser Line‐Scanning Confocal Fluorescence Imaging of the Photodynamic Action of Aluminum and Zinc Phthalocyanines in V79–4Chinese Hamster Fibroblasts
Author(s) -
Scully A. D.,
Ostler R. B.,
MacRobert A. J.,
Parker A. W.,
Lara C. de,
O'Neill P.,
Phillips D.
Publication year - 1998
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1998.tb02489.x
Subject(s) - confocal , fluorescence , confocal microscopy , fluorescence microscope , microscope , zinc , microscopy , materials science , fluorescence lifetime imaging microscopy , two photon excitation microscopy , laser , biophysics , chemistry , optics , biology , physics , metallurgy
Confocal fluorescence microscopy, using a newly constructed laser line‐scanning confocal microscope, was applied to an investigation of the early stages of photoinduced destruction of V79–4Chinese hamster fibroblasts using aluminum and zinc phthalocyanines as photosensitize. Results obtained in this work show that aluminum and zinc phthalocyanines, once internalized, localize in perinuclear sites that are disrupted upon light exposure resulting in fluorescence redistribution. The combination of laser‐line scanning with charge‐coupled device detection used in the confocal microscope developed in this work can enable rapid high‐resolution sequential imaging, which is ideal for studying photoinduced intracellular fluorescence dynamics.