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Blue Light‐induced Acidification of the Medium and the Uptake of Nitrate in a Nitrate‐starved Chlorella
Author(s) -
Kamiya Akio
Publication year - 1997
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1997.tb08657.x
Subject(s) - nitrate , staurosporine , chlorella , chemistry , phosphate , biochemistry , algae , botany , biology , protein kinase c , enzyme , organic chemistry
When cells of nitrate‐starved Chlorella kessleri (Fott et Novakova, no. 211‐11h) were suspended in phosphate buffer containing nitrate, they could barely take up nitrate in the dark. The addition of glucose resulted in the initiation of nitrate uptake by Chlorella. Irradiation with blue light stimulated the uptake of nitrate about two‐fold as compared with those of glucose‐treated cells and the acidification of the suspension medium, which, after 60‐90 min of blue irradiation, turned to alkalinization. These effects of blue light on the uptake of nitrate and pH changes were also observed in nitrate‐starved colorless (Fott et Novakova, no. 9.80) and yellow (Fott et Novakova, no. 211‐11h/20) mutants of C. kessleri. Staurosporine and K252a, potent inhibitors of protein kinase C, inhibited the blue light‐induced acidification of the medium and nitrate uptake. The data are discussed with regard to transduction of the signal for blue light‐induced acidification and nitrate uptake.