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Diamino Acid Derivatives of Porphyrins Penetrate into Yeast Cells, Induce Photodamage, but Have No Mutagenic Effect
Author(s) -
Zofadek Teresa,
Nhi Nguyen Bich,
Jagietto Izabella,
Graczyk Alfreda,
Rytka Joanna
Publication year - 1997
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1997.tb08651.x
Subject(s) - phototoxicity , yeast , saccharomyces cerevisiae , hematoporphyrin , porphyrin , chemistry , biochemistry , photosensitizer , amino acid , cell , photodynamic therapy , in vitro , photochemistry , organic chemistry
The yeast Saccharomyces cerevisiae was used as a model eukaryotic organism to study the uptake of diamino acid derivatives of porphyrins and their phototoxicity with particular emphasis on possible mutagenic effects. The water‐soluble hematoporphyrin derivatives diarginate (HpD[Arg] 2 ) and 1‐arginin di(N‐amino acid)‐protoporphyrinate used in this study are effective photosensitizers in tumor photodynamic therapy. Depending on the amino acid substituent, the porphyrin derivatives differ in their affinity for yeast cells. It is shown that HpD(Arg) 2 and PP(Met) 2 (Arg) 2 penetrate into the yeast cell and are metabolized. Both compounds sensitize yeast cells to photodamage but have no mutagenic effect on nuclear or mitochondrial genomes.