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The Role of Protein Kinase C Activity in the Killing of Chinese Hamster Ovary Cells by Ionizing Radiation and Photodynamic Treatment
Author(s) -
Rasch Monique H.,
Tijssen Karmi,
Lagerberg Johan W. M.,
Corver Wim E.,
Steveninck John,
Dubbelman Tom M. A. R.
Publication year - 1997
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1997.tb08645.x
Subject(s) - chinese hamster ovary cell , protein kinase c , cytotoxic t cell , ionizing radiation , microbiology and biotechnology , cytotoxicity , cell culture , hamster , chemistry , biology , context (archaeology) , protein kinase a , cancer research , in vitro , kinase , biochemistry , irradiation , genetics , paleontology , physics , nuclear physics
In several recent studies it has been shown that protein kinase C (PKC) activity may either potentiate or antagonize cell killing by different cytotoxic agents. These apparently conflicting observations suggest that the effects of PKC activity on cell survival may depend on the different properties of different cell types but do not exclude the possibility that the effects may also depend on the nature of the cytotoxic agent. In this context the effects of PKC activation and PKC inhibition or down‐regulation on Chinese hamster ovary (CHO) cell survival after photodynamic treatment and ionizing radiation were studied. It appeared that PKC activation by short‐term incubation with 12‐0‐tetradecanoyl‐phorbol‐13‐acetate (TPA) protected CHO cells against ionizing radiation but, in contrast, sensitized the cells to photodynamic treatment. Conversely, inhibition of PKC by H7 and down‐regulation of PKC activity by prolonged incubation with TPA sensitized CHO cells to ionizing radiation but protected the cells against photodynamic treatment. These results demonstrate that in one particular cell type PKC activity may have opposite effects on cell survival following cellular damage, depending on the nature of the cytotoxic agent .