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Potential Involvement of Both Type I and Type II Mechanisms in M13 Virus Inactivation by Methylene Blue Photosensitization
Author(s) -
Abe H.,
Ikebuchi K.,
Wagner S. J.,
Kuwabara M.,
Kamo N.,
Sekiguchi S.
Publication year - 1997
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1997.tb08644.x
Subject(s) - methylene blue , singlet oxygen , virus inactivation , chemistry , photochemistry , oxygen , irradiation , bacteriophage , azide , biophysics , virus , biochemistry , biology , virology , escherichia coli , catalysis , organic chemistry , photocatalysis , physics , gene , nuclear physics
We have investigated the mechanism of virus photoinactivation with methylene blue (MB) by conducting deuterium oxide (D 2 O), azide ion (N 3 ‐ ) and oxygen‐dependent, studies. Inactivation of M13 bacteriophage and singlet oxygen ( 1 O 2 ) generation by MB photosensitization were irradiation dose dependent. Inactivation of M13 was enhanced by D 2 O and inhibited by N 3 ‐ , suggesting that 1 O 2 participates in M13 inactivation by MB photosensitization. However, N 3 ‐ did not inhibit M13 inactivation completely. On the other hand, deoxygenating the reaction solution still caused 52‐67% of M13 inactivation observed in the presence of oxygen. These results suggest that 1 0 2 ‐mediated (Type II) and sensitizer‐mediated (Type I) reactions may both play roles in M13 inactivation by MB photosensitization.