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Magnetic Resonance Imaging Evaluation of Photodynamic Therapy‐Induced Hemorrhagic Necrosis in the Murine M1 Tumor Model
Author(s) -
Winsborrow BeAtrice G.,
Grondey Hiltrud,
Savoie Huguette,
Fyfe Colin A.,
Dolphin David
Publication year - 1997
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1997.tb03236.x
Subject(s) - photodynamic therapy , magnetic resonance imaging , necrosis , in vivo , pathology , medicine , nuclear medicine , nuclear magnetic resonance , radiology , chemistry , biology , physics , microbiology and biotechnology , organic chemistry
— Proton magnetic resonance imaging (MRI) and histological methods were used to evaluate photodynamic therapy (PDT)‐induced hemorrhagic necrosis in the murine Ml tumor within 72 h of treatment of male DBA/2 mice. The effects of three photosensitizing drugs were investigated: Photofrin (n = 4), Zn (II) phthalocyanine (n = 7) and benzoporphyrin derivative monoacid ring A (n = 11). As noted in previous studies of PDT using MRI, MRI makes possible serial, noninvasive, in vivo observation of tissue response to PDT. Our serial study of MRI and histological data confirms that tumors responded in the same way to PDT treatment using the three photosensitizing drugs: vascular damage followed by hemorrhagic necrosis. Most importantly and unlike previous MRI studies of PDT, we used a very high field magnet that enhanced the effect of magnetic susceptibility on image signal when blood is processed by the body after PDT‐induced hemorrhagic necrosis. This last finding demonstrates the utility of high field magnets and the importance of localized, serial experiments in future magnetic resonance studies of PDT.