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Photodynamic Efficiency of Protoporphyrin IX: Comparison of Endogenous Protoporphyrin IX Induced by 5‐Aminolevulinic Acid and Exogenous Porphyrin IX
Author(s) -
Tabata Kenji,
Ogura Shunichiro,
Okura Ichiro
Publication year - 1997
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1997.tb03235.x
Subject(s) - protoporphyrin ix , hela , porphyrin , chemistry , protoporphyrin , photodynamic therapy , membrane , incubation , mitochondrion , biochemistry , endogeny , in vitro , organic chemistry
— Photodynamic efficiency of protoporphyrin IX (PP) accumulated in HeLa cells by the incubation of PP with HeLa cells was compared with that of accumulated PP formed from 5‐aminolevulinic acid (ALA) as a precursor. The ALA‐induced PP was photodynamically more efficient than exogenous PP. The difference is caused by monomelic PP concentration and PP localization site in HeLa cells. Exogenous PP was accumulated mainly in plasma membrane, and the membrane was strongly damaged by irradiation. The ALA‐induced PP was selectively accumulated in mitochondria and inactivated the mitochondrial function by irradiation.