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Photoinactivation of Virus Infectivity by Hypocrellin A
Author(s) -
Hirayama Junichi,
Ikebuchi Kenji,
Abe Hideki,
Kwon KilWon,
Ohnishi Yoshiko,
Horiuchi Motohiro,
Shinagawa Morikazu,
Ikuta Kazuyoshi,
Kamo Naoki,
Sekiguchi Sadayoshi
Publication year - 1997
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1997.tb03209.x
Subject(s) - infectivity , vesicular stomatitis virus , virus , sodium azide , virology , chemistry , superoxide dismutase , biology , titer , microbiology and biotechnology , biochemistry , enzyme
— We investigated the photoinactivation of virus infectivity by hypocrellin A and its mechanism. The titers of vesicular stomatitis virus (VSV) and human immunodeficiency virus type 1 (HIV‐1), both of which are enveloped viruses, were reduced upon illumination with hypocrellin A in a concentration‐dependent manner, whereas canine parvovirus, a nonenveloped virus, was not killed. The removal of oxygen or addition of sodium azide or bT‐carotene both inhibited VSV inactivation. Mannitol and superoxide dismutase had no effect on VSV inactivation. These results indicate that singlet oxygen was involved in the process of VSV inactivation. Of the three major VSV membrane proteins, peripheral membrane protein M was most damaged by the hypocrellin A phototreatment.