Premium
Action Spectrum for Induction of Promoter Activity of Phenylalanine Ammonia‐Lyase Gene by UV in Carrot Suspension Cells
Author(s) -
Takeda Junko,
Ozeki Yoshihiro,
Yoshida Kazuichi
Publication year - 1997
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1997.tb03174.x
Subject(s) - promoter , action spectrum , phenylalanine ammonia lyase , photobiology , microbiology and biotechnology , electroporation , luciferase , gene , biology , daucus carota , transcription (linguistics) , reporter gene , chemistry , biochemistry , biophysics , gene expression , enzyme , transfection , botany , linguistics , philosophy , peroxidase
— The full‐length promoter (−2335) of the carrot ( Daucus carota ) phenylalanine ammonia‐lyase gene ( gDcPALl ) fused to the luciferase reporter gene was transiently transformed to carrot protoplasts by electroporation, and the promoter activity induced by monochromatic UV light of various wavelengths was examined. The action spectrum constructed from the fluence–response curves showed a single peak at around 280 nm, suggesting that the activation of the gDcPALl promoter is categorizable as one of the UVB light responses. The same assay system was applied to variously truncated gDcPALl promoters and to CaMV35S promoter fusion with various parts 5′‐ upstream of the gDcPALl promoter. The region from ‐396 to ‐190 (relative to the transcription start site) fused to the CaMV35S core (‐90) promoter showed a 280 nm‐dominant response. However, gDcPALl promoters truncated above ‐570 and ‐396, although they contain the region between ‐396 and ‐190, did not show such a typical UVB response, i.e. they responded to 260 nm light as much as to 280 nm light. The promoter truncated to below ‐190 also responded to 260 nm light as much as to 280 nm light. Therefore we assumed that the gDcPALl promoter is composed of three functionally different parts: the upstream above ‐570 (modulator), the region from ‐396 to ‐190 (UVB responsive) and the downstream below ‐190 (UVB and C responsive). The overall UVB response of the gDcPALl full‐length promoter is explained as the result of interaction of these three components.