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Effect of Photodynamic Pretreatment on the Susceptibility of Murine Tumor Cells To Macrophage Antitumor Mechanisms
Author(s) -
Reiter Isabella,
Schwamberger Giinter,
Krammer Barbara
Publication year - 1997
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1997.tb03162.x
Subject(s) - hematoporphyrin , phototoxicity , cytotoxicity , macrophage , tumor necrosis factor alpha , photodynamic therapy , in vitro , cytotoxic t cell , chemistry , cancer research , macrophage activating factor , immunology , microbiology and biotechnology , biology , biochemistry , organic chemistry
— In vitro photodynamic treatment of YAC‐1 murine T‐lymphoma cells with the hematoporphyrin derivative Photosan 3 and red light resulted in dose‐dependent phototoxicity. Photodynamic pretreatment, however, did not render these cells more susceptible to macrophage‐me‐diated tumor cytotoxicity or the cytotoxic effects of mac‐rophage‐derived antitumor mediators like tumor necrosis factor aL (TNF‐aL) or interferon bT (IFN‐bT). Independent of the degree of photosensitization used, the cytotoxicity values obtained with macrophages or the different mediators were shifted by the respective values for phototoxicity, suggesting these effects to be additive and thus not interdependent. These data show that while higher overall tumor cytotoxicity can be achieved by a combination of photodynamic treatment and macro‐phage‐mediated tumor destruction, this apparently is not a result of enhanced sensitivity of photodynamically treated tumor cells to macrophage antitumor mechanisms in general