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Inverse Filtering and Linear Prediction as New Analytical Approaches to Analyze the Change of Absorbance Spectra in Visible Spectroscopy: in vivo Study of Protochlorophyll(ide) Phototransformation
Author(s) -
Salvetat Richard,
Eullaffroy Philippe,
Popovic Radovan
Publication year - 1997
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1997.tb03153.x
Subject(s) - spectroscopy , spectral line , inverse , absorption spectroscopy , absorbance , analytical chemistry (journal) , chemistry , biological system , materials science , optics , mathematics , physics , chromatography , biology , geometry , quantum mechanics , astronomy
— The common approach of analyzing spectra by derivation is known to be inadequate for kinetic studies in visible spectroscopy (R. I. Sharger, Photochem. Photobiol. 38, 615‐617, 1983) because the side‐lobes height introduces artifacts and reduces readability in three‐dimensional graphic representation. In this study we present for the first time a parametric method that enables the use of inverse filtering and linear prediction for kinetic investigation in visible spectroscopy in cell biology studies. The protochlorophyll(ide) phototransformation at room temperature (295 K) was used to demonstrate the performance of this analytical approach. The change of absorption spectra in etiolated barley leaves was analyzed after being illuminated by a 50 ms flash.