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Identification of Two Palmitoyl Groups in Octopus Rhodopsin
Author(s) -
Nakagawa Masashi,
Iwasa Tatsuo,
Kikkawa Satoshi,
Takao Toshifumi,
Shimonishi Yasutsugu,
Tsuda Motoyuki
Publication year - 1997
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1997.tb01897.x
Subject(s) - rhodopsin , octopus (software) , chemistry , cleavage (geology) , peptide , fluorescence , mass spectrometry , fast atom bombardment , conjugated system , stereochemistry , biochemistry , chromatography , biology , retinal , organic chemistry , paleontology , physics , computational chemistry , quantum mechanics , fracture (geology) , polymer
— We determined the structure and site of fatty acid incorporated in octopus rhodopsin using a combination of fluorescence label and enzymatic cleavage methods in conjunction with fast‐atom bombardment (FAB) mass spectrometry. A single peptide containing two adjacent cysteines, Cys337 and Cys338, was successfully isolated using the fluorescence from a dye conjugated to Cys345. The FAB mass spectrometric analysis of the peptide (323phe‐ 340 phe) revealed that two palmitoyl groups are linked to Cys337 and Cys338 via thioester bonds in octopus rhodopsin as in bovine rhodopsin.