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Characterization of the Photosynthetic Activity of Platinized Chloroplasts: A Study Using Fluorescence and Photoacoustic Techniques
Author(s) -
Supponeva Elena,
Hotchandani Surat,
Carpentier Robert
Publication year - 1996
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1996.tb09639.x
Subject(s) - photosystem ii , photochemistry , photosynthesis , chemistry , oxygen evolution , electron transport chain , chlorophyll fluorescence , bicarbonate , light harvesting complexes of green plants , photosystem i , electron acceptor , oxygen , fluorescence , chloroplast , photoacoustic spectroscopy , biophysics , photoacoustic imaging in biomedicine , biochemistry , biology , optics , physics , organic chemistry , electrode , electrochemistry , gene
In this report, the effect of platinization on the photosynthetic activity of the chloroplast membranes is studied. Oxygen evolution, fluorescence emission and thermal de‐activation processes are modified after platinization. It is shown that photosystem II activity is affected by the hydrogen purging involved in the platinization procedure as seen by the reduced rates of oxygen evolution and a decrease in variable fluorescence. Depletion of bicarbonate from photosystem II during purging is suggested to be responsible partly for the decreased electron transfer rates and for a lower half‐saturation light intensity required for energy storage as measured by photoacoustic spectroscopy. On the other hand, the electron sink created by the reduction of hydrogen at the acceptor side of photosystem I is shown to reoxidize efficiently the plas‐toquinone pool of photosystem II.