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Delayed Oxidative Photodamage induced by Photodynamic Therapy
Author(s) -
Kessel David,
Luo Yu
Publication year - 1996
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1996.tb03111.x
Subject(s) - phototoxicity , photodynamic therapy , apoptosis , trolox , chemistry , dna fragmentation , fragmentation (computing) , oxidative phosphorylation , scavenger , irradiation , dna damage , leukemia , photosensitizer , cancer research , photochemistry , microbiology and biotechnology , in vitro , oxidative stress , dna , radical , biochemistry , programmed cell death , biology , immunology , ecology , physics , organic chemistry , nuclear physics , antioxidant capacity
— Apoptotic DNA fragmentation was observed 60 min after photodynamic therapy of murine leukemia cells in culture, using either of two photosensitizers with predominantly lysosomal targets. When the radical scavengers trolox or α‐tocopherol succinate were present during irradiation, the subsequent appearance of apoptotic cells was prevented, as was phototoxicity. Addition of either scavenger during the 60 min after irradiation provided only partial protection from apoptosis and phototoxicity; this protection was abolished if the addition was delayed for 10 min. These results are consistent with a model whereby long‐persisting photoproducts continue the initiation of apoptosis for approximately 10 min after irradiation has ceased.