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Replication in UV‐lrradiated Caenorhabditis elegans Embryos
Author(s) -
Jones Clark A.,
Hartman Phil S.
Publication year - 1996
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1996.tb03012.x
Subject(s) - embryo , caenorhabditis elegans , biology , mutant , dna replication , dna synthesis , dna damage , elongation , dna , wild type , microbiology and biotechnology , genetics , gene , materials science , ultimate tensile strength , metallurgy
— Replication continues in wild‐type (but not rad mutant) Caenorhabditis elegans embryos even after exposure to massive fluences of UV radiation. It is of interest to elucidate the mechanism(s) for this “damage‐resistant” DNA synthesis. In this study, DNA from unirradiated and UV‐irradiated wild‐type embryos was examined using the electron microscope. Large fluences of UV radiation (180 J m −2 ) had little effect on either replication bubble size or distances between bubbles in wild‐type embryos, indicating that the damage‐resistant DNA synthesis was not grossly aberrant. Conversely, UV irradiation significantly decreased center‐to‐center distances between bubbles in excision‐repair‐deficient rad‐3 embryos. This suggests that the decreased DNA synthesis observed after UV irradiation in rad‐3 embryos is due largely to blockage of elongation of DNA synthesis.