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Protochlorophyllide Reductase III: Synthesis of a Protochlorophyllide‐Dihydroflavin Complex
Author(s) -
Nayar Parmesh,
Begley Tadhg P.
Publication year - 1996
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1996.tb02998.x
Subject(s) - protochlorophyllide , chemistry , moiety , quenching (fluorescence) , reductase , fluorescence , enzyme , biochemistry , photochemistry , stereochemistry , oxidoreductase , physics , quantum mechanics
— A mild and efficient method of linking a dihydroflavin to the C‐17 carboxylic acid side chain of protochlorophyllide, without degradation of the sensitive E ring or loss of magnesium, is described. The appended dihydroflavin was shown to quench the fluorescence of protochlorophyllide. In contrast, a dihydronicotinamide moiety was unable to effect fluorescence quenching. The relevance of these findings to a possible mechanism of action of the enzyme protochlorophyllide reductase is discussed.