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Phospholipases Induce Melanogenesis in Organ‐Cultured Skin
Author(s) -
Maeda* Kazuhisa,
Tomita Yasushi,
Naganuma Masako,
Tagaml Hachiro
Publication year - 1996
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1996.tb02446.x
Subject(s) - melanin , tyrosinase , organ culture , phosphatidylinositol , arachidonic acid , biology , microbiology and biotechnology , hyperpigmentation , stimulation , endocrinology , signal transduction , chemistry , biochemistry , enzyme , in vitro
Guinea pig skin becomes more pigmented following exposure to UV rays. This melanization was accompanied by enhanced intensity of tyrosinase‐staining and increased number of tyrosinase‐positive melanocytes (MEL ty+ ), with resultant enhancement of melanin synthesis. To clarify the regulatory mechanism for melanization following UV irradiation, organ‐cultured guinea pig skins have been used to examine their melanogenic responses to exogenous stimulation. This organ culture system responded well to UV irradiation, by increasing melanogenic activity. Also, in this system, phospholipases (PL), arachidonic acid, interleukin‐1α and melanocytestimulating hormone, but not endothelin‐1 or phosphatidylinositol‐specific PLC (PI‐PLC), stimulated melanogenesis to various extents as indicated by the number of MEL ty+ and morphological changes. Among them, the PLA 2 and PLD were found to have a potent stimulatory property for melanocytes. They might affect melanocytes directly or indirectly through an effect on keratinocytes. These results suggest that PLA 2 and PLD play a key role in epidermal hyperpigmentation after UV irradiation or inflammation.