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Solar‐simulated Ultraviolet Irradiation Induces Selective Influx of CD4+ T Lymphocytes in Normal Human Skin
Author(s) -
Nuzzo Sergio Di,
Rie Menno A.,
Loos Chris M.,
Bos Jan D.,
Teunissen Marcel B. M.
Publication year - 1996
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1996.tb01866.x
Subject(s) - cd3 , human skin , epidermis (zoology) , cd8 , dermis , population , erythema , staining , infiltration (hvac) , microbiology and biotechnology , chemistry , irradiation , andrology , biology , immunology , pathology , immune system , anatomy , medicine , materials science , genetics , environmental health , composite material , physics , nuclear physics
— The proportion and composition of the human cutaneous CD3+ T lymphocyte population was determined in situ following a single exposure to physiological, erythema‐inducing doses of simulated solar radiation, mainly consisting of UV radiation. Biopsies were taken 1, 2 and 7 days after local irradiation of normal volunteers with 1,2 and 4 MED by a xenonarc lamp and immunohistochemistry was performed on cryostat sections. Ultraviolet radiation caused an initial decrease of intraepidermal CD3+ T‐cell numbers or even could lead to T‐cell depletion 24 and 48 h postirradiation, and this was followed by an infiltration of T cells in the epidermis as determined 1 week after UV exposure. The number of dermal CD3+ T ceDs was increased 24 h after irradiation, reached a maximum at 48 h and subsequently declined at day 7, though remained significantly higher than the unirradiated control Double staining demonstrated that the CD3+ T cells, which immigrated into the (epi)dermis upon UV exposure, coexpressed CD4 but not CD8. Therefore the CD4/CD8 ratio in skin was markedly increased during the first week upon UV exposure. Our time course study shows that UV radiation affects die T‐cell population within human skin by depleting the majority of epidermal T cells and initiating a selective influx of CD4+ T cells.