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Confocal Fluorescence Lifetime Imaging of Chlorophyll Molecules in Polymer Matrices
Author(s) -
Sanders R.,
Zandvoort M. A. M. J.,
Draaijer A.,
Levine Y. K.,
Gerritsen H. C.
Publication year - 1996
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1996.tb01840.x
Subject(s) - polyvinyl alcohol , fluorescence , confocal , polymer , fluorescence lifetime imaging microscopy , nitrocellulose , molecule , matrix (chemical analysis) , chemistry , materials science , photochemistry , analytical chemistry (journal) , optics , chromatography , physics , organic chemistry , biochemistry , membrane
— Confocal fluorescence lifetime imaging has been used to establish the microscopic disposition of chlorophyll molecules in polymer matrices. This study provides a direct test of models proposed on the basis of fluorescence measurements on bulk Alms. We show that whereas chlorophyll b molecules are uniformly distributed in nitrocellulose matrices, they form pocket‐like structures in polyvinyl alcohol matrices. The shape of the structures, seen as bright fluorescent spots on a uniform background, is modified on stretching the polyvinyl alcohol matrix.