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Use of Uracil Thin Layer for Measuring Biologically Effective UV Dose
Author(s) -
Gróf Pal,
Gáspái Sándor,
Rontó Györgyi
Publication year - 1996
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1996.tb01837.x
Subject(s) - dosimeter , uracil , irradiation , chemistry , absorption (acoustics) , dosimetry , materials science , radiation , optoelectronics , radiochemistry , analytical chemistry (journal) , optics , nuclear medicine , chromatography , physics , biochemistry , dna , nuclear physics , medicine
— Dimerization of uracil monomers in a polycrystalline state by UV radiation changes the absorption characteristics of a thin layer of the material. The change in optical density, measured by spectrophotometry in the250–400 nm range, as a function of the exposure time is evaluated in terms of the biologically effective UV dose. A statistical evaluation of a great number of uracil dosimeters irradiated with a TL01 lamp from Philips establishes the possibility of evaluating the biologically effective UV dose using a uracil dosimeter. Nonlinear regression procedures were introduced to correct the absorption spectra for contributions due to light scattering and to determine the optical density values required to calculate the UV dose expressed in H U units. Comparison of cumulative daily doses and long‐term monitoring measured by the uracil thin‐layer dosimeter and a phage T7 dosimeter are given, which allow the determination of conversion factors between various biological dosimeters under different irradiation conditions.