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INTERACTIONS BETWEEN N ‐ASPARTYL CHLORIN E6, DETERGENT MICELLES AND PLASMA LIPOPROTEINS
Author(s) -
Kessel David
Publication year - 1995
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1995.tb09882.x
Subject(s) - chemistry , quenching (fluorescence) , chlorin , micelle , cationic polymerization , photochemistry , fluorescence , molecule , porphyrin , organic chemistry , aqueous solution , physics , quantum mechanics
Addition of plasma to the photosensitizing agent N ‐aspartyl chlorin e6 (NPe6) in Triton X‐100 micelles results in a blue‐shift of fluorescence emission, corresponding to a change in the average environmental dielectric constant from 8 to 32. This effect was not observed with anionic or cationic detergents or with several other sensitizers including a close analog, chlorin e6. The apolipoprotein fraction of high‐density lipoproteins (HDL) was found responsible for this blue‐shift. These results indicate a highly selective interaction between HDL apolipoproteins and NPe6 molecules. Addition of the HDL apolipoprotein to NPe6 incorporated into Triton micelles decreased the fraction of NPe6 molecules accessible to Cd 2+ ‐induced fluorescence quenching but resulted in an increase in the Stern‐Volmer quenching constant (an index of quenching efficiency).