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UPTAKE KINETICS AND INTRACELLULAR LOCALIZATION OF HYPOCRELLIN PHOTOSENSITIZERS FOR PHOTODYNAMIC THERAPY: A CONFOCAL MICROSCOPY STUDY
Author(s) -
Miller Gerald G.,
Brown Kevin,
Moore Ronald B.,
McPhee Malcolm S.,
Diwu Zhenjun J.,
Liu Jixiang,
Huang Liren,
Lown J. William,
Begg David A.,
Chlumecky Vera,
Tulip John
Publication year - 1995
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1995.tb09880.x
Subject(s) - confocal microscopy , kinetics , phototoxicity , golgi apparatus , intracellular , photodynamic therapy , confocal , chemistry , photosensitizer , endoplasmic reticulum , biophysics , fluorescence , fluorescence microscope , biochemistry , microbiology and biotechnology , photochemistry , biology , in vitro , physics , geometry , mathematics , organic chemistry , quantum mechanics
Hypocrellins are naturally occurring compounds with photosensitizing properties in biological systems. We have prepared synthetic derivatives of hypocrellin B, which have promise as photosensitizers in the clinical application of photodynamic therapy. The intracellular localization and uptake kinetics of hypocrellin B and several selected hypocrellin congeners were determined semiquantitatively by fluorescence confocal microscopy in monolayer cultures of EMT6/Ed murine tumor cells. Each compound had unique uptake kinetics. Although no compound tested to date has demonstrated nuclear labeling, most could be detected in lysosomes, Golgi, endoplasmic reticulum and, to a minor extent, in cellular membranes. No two compounds gave identical labeling distributions. The differences are assumed to originate in physicochemical properties characteristic of each compound, which may ultimately impact upon the primary modality of phototoxicity.