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PHOTOCHEMICAL LINKAGE OF ANTIBODIES TO SILICON CHIPS
Author(s) -
Sundarababu Gajendran,
Gao Hui,
Sigrist Hans
Publication year - 1995
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1995.tb09867.x
Subject(s) - chemistry , covalent bond , silicon , adsorption , bovine serum albumin , photochemistry , aqueous solution , monoclonal antibody , molecule , aqueous medium , irradiation , antibody , chromatography , organic chemistry , physics , nuclear physics , immunology , biology
Antibodies and antigen binding fragments thereof were photochemically immobilized on surface‐modified silicon chips of 5 × 5 mm size. Silicon surface‐grafted diazirines and benzophenones formed covalent bonds with the immunoreagents on light activation. Photolithographic immobilization of monoclonal antibodies in aqueous media was achieved on silicon chips by activating surface‐grafted benzophenones. The presence of bovine serum albumin during irradiation reduced nonspecific adsorption of the immunoreagents and retained the immunoactivity of the photoimmobilized molecules.