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TEMPERATURE DEPENDENCE OF CHLOROPHYLL(IDE) SPECTRAL SHIFTS and PHOTOACTIVE PROTOCHLOROPHYLLIDE REGENERATION AFTER FLASH IN ETIOLATED BARLEY LEAVES
Author(s) -
Eullaffroy Philippe,
Salvetat Richard,
Franck Fabrice,
Popovic Radovan
Publication year - 1995
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1995.tb08725.x
Subject(s) - protochlorophyllide , etiolation , absorbance , chlorophyll , chemistry , pigment , photochemistry , botany , biophysics , biology , biochemistry , chloroplast , enzyme , chromatography , organic chemistry , gene
— Absorbance spectroscopy at 77 K was used to investigate the effect of temperature on in vivo chlorophyllide shifts and photoactive protochlorophyllide regeneration after a saturating flash, which transformed all protochlorophyllide to chorophyllide. Photoactive protochlorophyllide present in darkness was stable up to 40°C. The rate of Shibata shift and protochlorophyllide regeneration after flash were strongly temperature dependent in the range 0–25°C. At 0°C, the shift was still observed but no regeneration occurred. Only slight effects were observed in the range 25–40°C. At all temperatures, the process of protochlorophyllide regeneration was significantly slower than the Shibata shift. The final chlorophyll shift from 672 to 674 nm was observed up to 40°C. The implication of these results concerning the pigment‐protein interactions during the Shibata shift are discussed.