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ANALYSIS OF LASER‐INDUCED FLUORESCENCE LINE SHAPE OF INTACT LEAVES: APPLICATION TO UV STRESS DETECTION
Author(s) -
Subhash N.,
Azzinghi Piero M,
Agati Giovanni,
Fusi Franco,
Lercari Bartolomeo
Publication year - 1995
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1995.tb08720.x
Subject(s) - fluorescence , spectral line , laser , excited state , laser induced fluorescence , analytical chemistry (journal) , chemistry , emission spectrum , materials science , optics , atomic physics , physics , chromatography , astronomy
—In vivo laser‐induced fluorescence spectra of intact leaves of healthy and UV‐irradiated Salvia splendens plants excited at 337 nm by a nitrogen laser were recorded using an optical multichannel analyzer system. The spectra showed the typical fluorescence bands centered around 450, 530, 685 and 730 nm. Exposure to UV radiation changed the relative intensity values of these bands and their peak positions. The analysis of the acquired spectra in terms of a linear combination of Gaussian bands was carried out to determine accurately the peak positions and the relative intensity contribution of the various bands to the laser‐induced fluorescence spectra on healthy and UV‐treated plants of different age. The results indicate that a curve‐fitting analysis of the measured fluorescence spectra is a useful and sensitive method to discriminate the various band contribution to the whole leaf fluorescence spectrum. The comparison among blue‐green and red‐far‐red fluorescence of leaves was also confirmed as an effective indicator of UV stress in plants.