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A METHOD FOR THE ELECTROCHEMICAL INITIATION OF THE in vitro BACTERIAL LUCIFERASE REACTION
Author(s) -
Karatani Hajime,
Shizuki Takahiro,
Halon Rozana,
Nakayama Eiichiro
Publication year - 1995
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1995.tb08634.x
Subject(s) - luciferase , vibrio harveyi , chemistry , riboflavin , redox , in vitro , electrochemistry , photochemistry , biochemistry , vibrio , inorganic chemistry , bacteria , electrode , biology , transfection , genetics , gene
— We have developed an electrochemical initiation method for the in vitro bacterial luciferase reaction using Vibrio harveyi luciferase. The cathodic conversion of oxidized riboflavin 5′‐phosphate (FMN) to its reduced form (FMNH 2 ), on which the kinetics for the overall light‐emitting reaction depend, is followed by the bacterial luciferase reaction. The electrochemical initiation method can be used for both turnover and nonturnover luciferase reactions, depending on the FMN electroreduction methods.