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CLONING, EXPRESSION and SEQUENCE ANALYSIS OF cDNA FOR THE LUCIFERASES FROM THE JAPANESE FIREFLIES, Pyrocoelia tniyako AND Hotaria parvula
Author(s) -
Ohmiya Yoshihiro,
Ohba Nobuyoshi,
Toh Hiroyuki,
Tsuji Frederick I.
Publication year - 1995
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1995.tb05273.x
Subject(s) - luciferase , luciferases , complementary dna , biology , peptide sequence , genbank , microbiology and biotechnology , homology (biology) , nucleic acid sequence , amino acid , sequence analysis , biochemistry , gene , transfection
— Cloning and sequence analysis of cDNA for the luciferases of Pyrocoelia miyako and Hotaria parvula were carried out (GenBank accession numbers L39928 and L39929, respectively). The amino acid sequence, deduced from the nucleotide sequence, showed P. miyako luciferase to consist of 548 amino acid residues with a molecular weight of 60955, while the luciferase of H. parvula consisted of 548 amino acid residues with a molecular weight of 60 364. Pyrocoelia miyako luciferase showed 82.1 % homology with the luciferase of Photinus pyralis and less than 70% homology with other firefly luciferases, whereas H. parvula luciferase showed 98%, 82.5% and 81.2% homology with the luciferases of Luciola mingrelica, Luciola lateralis and Luciola cruciata, respectively. Two regions in the enzymes were found to be highly conserved. The amino acid sequences were used to construct a phylogenetic tree, which showed that the fireflies could be divided into two groups.