Premium
COMPARISON OF THE SOLID‐MATRIX LUMINESCENCE PROPERTIES OF BENZO(a)PYRENE‐DNA ADDUCTS ON α‐CYCLODEXTRIN/NaCl and TREHALOSE/NaCl MATRICES
Author(s) -
Chu Yu,
Hurtubise Robert J.
Publication year - 1995
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1995.tb05266.x
Subject(s) - chemistry , trehalose , adduct , luminescence , pyrene , matrix (chemical analysis) , fluorescence , dna , oligonucleotide , chromatography , organic chemistry , biochemistry , materials science , physics , optoelectronics , quantum mechanics
Abstract— The solid‐matrix luminescence properties of (±)‐ trans ‐7,8‐dihydroxy‐anti‐9,10‐epoxy‐7,8,9,10‐tetrahydrobenzo(a)pyrene‐DNA ([±]‐anti‐BPDE‐DNA) adducts were compared on α‐cyclo‐dextrin (CD)/NaCl and trehalose/NaCl solid matrices. Both the optimum composition for the solid matrices and the best solvent system were obtained experimentally for acquiring the maximum room‐temperature fluorescence (RTF) and room‐temperature phosphorescence (RTP) signals for the (±)‐ anti ‐BPDE‐DNA. Most of the solid‐matrix RTF and RTP data were obtained at 296 K and 93 K for (±)‐anti‐BPDE‐DNA adducts adsorbed on 1%α‐CD/NaCl and 80% trehalose/NaCl. The RTF signals were strong for (±)‐BPDE‐DNA adducts on both solid matrices, but RTP was only obtained on the trehalose/NaCl solid matrices with the 80% trehalose yielding the strongest RTP signal for (±)‐anti‐BPDE‐DNA. The fluorescence lifetime data for (±)‐anti‐BPDE‐DNA gave two components on 1 %α‐CD/NaCl. For 80% trehalose/NaCl, three components were revealed, but two components were obtained with 80% trehalose/NaCl after ether extraction of the solid matrix. The third component was ascribed to the formation of the tetrols from (±)‐ anti ‐BPDE‐DNA adducts during the drying step in the sample preparation of 80% trehalose/NaCl. The results give the first reported data on the solid‐matrix luminescence of the (±)‐ anti ‐BPDE‐DNA adducts. These results should be of considerable interest not only from an analytical viewpoint but as a new means of studying the luminescence characteristics of the adducts.