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SEQUENCE SPECIFICITY OF TETRAHYDROBENZOPSORALEN PHOTOBINDING TO DNA
Author(s) -
Mobilio S.,
Tondelli L.,
Capobianco M.,
Gia O.
Publication year - 1995
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1995.tb03948.x
Subject(s) - bifunctional , dna , sequence (biology) , chemistry , oligonucleotide , non covalent interactions , covalent bond , base pair , reaction rate constant , covalent binding , dna sequencing , stereochemistry , kinetics , organic chemistry , biochemistry , molecule , hydrogen bond , physics , quantum mechanics , catalysis
— The sequence specificity of photobinding to DNA of two tetrahydrobenzopsoralen derivatives has been investigated by testing the photoreactivity toward a number of self‐complementary oligonucleotides. The thermodynamic constant for noncovalent binding to each DNA sequence was evaluated. The extent of photoreactivity was greatly dependent upon base composition. The two tetracyclic compounds show similar behavior in comparison to other bifunctional derivatives. Their overall rate constants were greatly enhanced in comparison to classical psoralens. However, their high efficiency of covalent binding is counterbalanced by low affinity for noncovalent interaction with DNA.