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In vitro DEMONSTRATION OF A FUNDAMENTAL DIFFERENCE IN THE PROLIFERATION OF MURINE and HUMAN BONE MARROW and LYMPHOCYTES FOLLOWING ULTRAVIOLET IRRADIATION: RELEVANCE TO BONE MARROW TRANSPLANTATION
Author(s) -
Hudson J. G.,
Pullens R.,
Godwin V.,
Preece A. W.,
Pamphilon D. H.
Publication year - 1995
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1995.tb02386.x
Subject(s) - human bone , bone marrow , in vitro , bone marrow transplantation , transplantation , immunology , irradiation , relevance (law) , cancer research , biology , chemistry , microbiology and biotechnology , medicine , biochemistry , physics , political science , nuclear physics , law
— Exposure of rodent allogeneic donor marrow and splenocyte grafts to ultraviolet radiation (UVR) has been shown to permit durable engraftment at doses that abolish graft‐ versus ‐host disease (GVHD) and graft rejection. We have compared both murine and human alloreactive and mitogen‐induced lymphoid responses and bone marrow proliferation in mixed lymphocyte culture (MLC), phy‐tohemagglutinin (PHA)‐induced proliferation and colony‐forming unit‐granulocyte/macrophage (CFU‐GM) assays using germicidal UVC (200–290nm), broadband and narrowband UVB (290–320nm) and UVA (320^100 nm) sources. Our data show a wavelength and dose‐dependent reduction in lymphoid proliferation in the mouse with CFU‐GM survival of50–75% of control at doses required to abolish allogeneic lymphocyte responses for all lamps. In contrast, human lymphocyte responses are more resistant to UVC with CFU‐GM proliferation reduced to zero when allostimulation is abolished. Mito‐gen‐induced lymphoid responses show a similar wavelength‐dependent sensitivity. Abolition of response in MLC using UV‐irradiated stimulator cells was less sensitive than proliferation with UV‐irradiated responder cells at all wavelengths in both species. With all sources, murine CFU‐GM proliferation is less susceptible to UVR than human marrow at doses required to abolish lymphoid responses.