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THE EFFECT OF PHOTODYNAMIC TREATMENT OF YEAST WITH THE SENSITIZER CHLOROALUMINUM PHTHALOCYANINE ON VARIOUS CELLULAR PARAMETERS
Author(s) -
Paardekooper Michel,
Gompel Angeline E. Van,
Steveninck John Van,
Broek Peter J. A. Van den
Publication year - 1995
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1995.tb02385.x
Subject(s) - chemistry , kluyveromyces marxianus , photodynamic therapy , biophysics , substrate (aquarium) , atpase , yeast , incubation , biochemistry , enzyme , biology , organic chemistry , saccharomyces cerevisiae , ecology
— Photodynamic treatment of Kluyveromyces marxianus with chloroaluminum‐phthalocyanine resulted in loss of clonogenicity. Several parameters were studied to identify targets that could be related to loss of colony‐forming capacity. Inhibition of various plasma membrane‐bound processes was observed, such as substrate transport and plasma membrane ATPase activity. Moreover, K+ loss from the cells was observed. Photodynamic treatment also reduced the activity of various enzymes involved in energy metabolism, thereby decreasing the cellular ATP level. It will be discussed however that none of these processes is likely to be related directly to loss of clonogenicity. Treatment with phthalocyanine and light resulted in a strong inhibition of the incorporation of 14C‐phenylalanine in trichloracetic acid‐precipitable material. The induction of the β‐galactoside utilization system was also strongly inhibited. The latter two processes did not recover during incubation, subsequent to photodynamic treatment. It is concluded that photodynamically induced inhibition of protein synthesis is a critical factor contributing to the loss of clonogenicity.