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PREFERENTIAL INHIBITION OF NUCLEOSOME ASSEMBLY BY ULTRAVIOLET‐INDUCED (6‐4)PHOTOPRODUCTS
Author(s) -
Matsumoto Hideki,
Takakusu Akira,
Mori Toshio,
Ihara Makoto,
Todo Takeshi,
Ohnishi Takeo
Publication year - 1995
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1995.tb02345.x
Subject(s) - nucleosome , pyrimidine dimer , cyclobutane , pbr322 , linker dna , chemistry , dna , biophysics , ultraviolet light , in vitro , photochemistry , chromatosome , plasmid , stereochemistry , histone , dna repair , biochemistry , biology , ring (chemistry) , organic chemistry
We reconstituted nucleosomes in vitro using two kinds of damaged pBR322 plasmid DNA carrying cyclobutane pyrimidine dimers (CPD) or (6‐4)photoproducts. The results indicate that nucleosome assembly is inhibited preferentially by (6‐4)photoproducts compared with CPD, suggesting that the regions carrying (6‐4)photoproducts retain their nucleosome‐free form, i.e. linker‐like conformation until completion of the repair processes.