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RESEARCH NOTE: THIOPYRONINE‐AND 8‐METHOXYPSORALEN‐SENSITIZED PHOTODYNAMIC EFFECT ON CELL GROWTH, COLONY FORMING ABILITY AND RNA SYNTHESIS IN Saccharomyces MUTANTS DEFICIENT IN DNA REPAIR
Author(s) -
Roth Robert M.,
Takamori Yasuhiko,
Lochmann ErnstRandolf
Publication year - 1994
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1994.tb09667.x
Subject(s) - mutant , dna repair , dna , dna damage , ultraviolet light , biology , microbiology and biotechnology , rna , mutation , chemistry , genetics , gene , photochemistry
We compared the photodynamic effects of thiopyronine (TP) and visible light, and 8‐methoxypsoralen (8‐MOP) and ultraviolet A (UV‐A) light, on growth, colony forming ability and RNA synthesis in a repair‐proficient Saccharomyces strain and three mutants deficient in DNA repair mechanisms (DNA repair assays). With 8‐MOP and UV‐A repair‐deficient mutants were significantly more sensitive than the repair‐proficient strain indicating that the system is sensitive for the detection of DNA damage. With TP and visible light, the photodynamic effects were comparable in the mutants and the control, indicating no DNA damage. These results support previous work showing that the main target of TP photosensitization in eukaryotes is not nuclear DNA.