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ABSORPTION SPECTRUM OF HEMATOPORPHYRIN DERIVATIVE in vivo IN A MURINE TUMOR MODEL
Author(s) -
Cubeddu Rinaldo,
Canti Gianfranco,
Musolino Mario,
Pifferi Antonio,
Taroni Paola,
Valentini Gianluca
Publication year - 1994
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1994.tb05151.x
Subject(s) - hematoporphyrin , in vivo , absorption (acoustics) , chemistry , analytical chemistry (journal) , nuclear magnetic resonance , materials science , chromatography , photodynamic therapy , physics , biology , microbiology and biotechnology , organic chemistry , composite material
‐Time‐resolved reflectance was used to measure the absorption spectrum of hematoporphyrin derivative (HpD) in vivo in a murine tumor model. Reflectance measurements were performed in the 600–640 nm range on mice bearing the L1210 leukemia. Then the animals were administered 25 mg/kg body weight of HpD intraperito‐neally. One hour later the reflectance measurements were repeated. Fitting of the data using the diffusion theory allowed assessment of the absorption coefficient before and after the administration. As a difference between the latter and the former data, the in vivo absorption spectrum of HpD was evaluated. Maximum absorption was measured at 620–625 nm. Similar spectral behavior was obtained for HpD in solution in the presence of low‐density lipoproteins.