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HEMATOPORPHYRIN DERIVATIVE (PHOTOFRIN®) PHOTODYNAMIC ACTION ON Ca 2+ TRANSPORT IN MONKEY KIDNEY CELLS (CV‐1)
Author(s) -
Dellinger Marc,
Ricchelli Fernanda,
Moreno Giuliana,
Salet Christian
Publication year - 1994
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1994.tb05117.x
Subject(s) - digitonin , endoplasmic reticulum , hematoporphyrin , intracellular , cytosol , mitochondrion , biophysics , chemistry , incubation , photodynamic therapy , respiration , biochemistry , biology , anatomy , enzyme , organic chemistry
After 24 h incubation with Photofrinm ® (PF), photodynamic action has been studied on Ca 2+ transport in CV‐1 cells. A moderate increase of the cytosolic free Ca 2+ concentration [Ca 2+ ] is observed immediately after a dose of irradiation which yields a survival rate of less than 5% at 48 h. In parallel, studies on digitonin‐permeabilized cells indicate that such a treatment inhibits endoplasmic reticulum Ca 2+ uptake with few alterations of this process in mitochondria. In contrast, ADP‐stimulated respiration is impeded and intracellular ATP level decreases. It is suggested that direct damage to endoplasmic reticulum as well as mitochondrial disturbance are the primary mechanisms responsible for a nontransient elevation of [Ca 2+] i preceding cell death.