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PHOTOACI'IVATED GILVOCARCIN V INDUCES DNA‐PROTEIN CROSSLINKING IN GENES FOR HUMAN RIBOSOMAL RNA AND DIHYDROFOLATE REDUCTASE
Author(s) -
Matsumoto Akira,
Fujiwara Yoshisada,
Elespuru Rosalie K.,
Hanawalt Philip C.
Publication year - 1994
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1994.tb05095.x
Subject(s) - dihydrofolate reductase , gene , ribosomal rna , dna , rna , microbiology and biotechnology , ribosomal protein , biochemistry , chemistry , biology , ribosome
The nature of DNA interactions with photoactivated gilvocarcin V has been analyzed at the gene level in both rRNA and dihydrofolate reductase genes of human fibroblasts, utilizing a modified Southern hybridization technique. Neither interstrand DNA crosslinking nor RNA linkage to DNA was detected. However, we consistently observed in both genes retarded DNA bands appearing in a dose‐dependent fashion following exposure to photoactivated gilvocarcin V. These retarded bands were enhanced when genomic DNA was prepared without proteinase K treatment, suggesting involvement of protein in this DNA interaction. Because these bands disappear following proteinase K treatment, it is probable that photoactivated gilvocarcin V induces DNA‐protein crosslinking.