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A FULLY AUTOMATED DUAL‐WAVELENGTH PHOTOMETER FOR PHYTOCHROME MEASUREMENTS AND ITS APPLICATION TO PHYTOCHROME FROM CHLOROPHYLLCONTAINING EXTRACE
Author(s) -
Lamparter Tilman,
Hughes Jon,
Hartmann Elmar
Publication year - 1994
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1994.tb05088.x
Subject(s) - phytochrome , photometer , wavelength , chlorophyll , optics , biology , monochromatic color , phase (matter) , botany , biophysics , chemistry , physics , red light , organic chemistry
The photometer described here measures phytochrome photoreversibility of as many as 20 samples fully automatically. Monochromatic light of two laser diodes (670 and 780 nm) is used as a source for the measuring light beam. The whole measuring procedure is controlled by a computer linked to a lock‐in amplifier. This arrangement permits a rather simple construction and allows automatic zeroing and sample change. In addition the application of a Triton phase partitioning method to allow phytochrome photoreversibility mea surements of extracts of green tissue is described. A single phase partitioning with a chlorophylYphytochrome mixture reduced chlorophyll concentration to less than 10%. Repeated phase partitioning of crude extracts from green moss protonemata reduced chlorophyll content to less than 2% while leaving the phytochrome concentration almost unchanged. In some instances this method has advantages over polyethyleneimine precipitation, which is commonly used to obtain chlorophyll‐free extracts.