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SITES OF PHOTODYNAMICALLY INDUCED DNA REPAIR IN HUMAN CELLS
Author(s) -
Kvam Egil,
Stokke Trond
Publication year - 1994
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1994.tb05061.x
Subject(s) - proliferating cell nuclear antigen , porphyrin , cytoplasm , dna , chemistry , biophysics , monoclonal antibody , dna damage , membrane , cell , macromolecule , cell nucleus , nuclear dna , microbiology and biotechnology , biochemistry , antibody , biology , genetics , gene , mitochondrial dna
Human REH cells were incubated with the photosensitizers meso ‐tetra(4‐sulfonatophenyl)porphyrin (TSPP=TPPS 4 ) or meso ‐tetra(3‐hydroxyphenyl)porphyrin (3‐THPP). The relatively hydrophilic TSPP was partly found in the cytoplasm and partly in the nuclei, whereas the lipophilic 3‐THPP was found apparently in membranes and not inside the nuclei. After illumination, sites of DNA repair were labeled by means of a monoclonal antibody against proliferating cell nuclear antigen (PCNA) bound in the nuclei. The amount of bound PCNA in non‐S‐phase cells was proportional to the light dose. The bound PCNA was homogeneously distributed in the nuclei 0.5 h after photodynamic treatment (PDT) with TSPP. In contrast, for cells given PDT with 3‐THPP, the periphery of the nuclei was selectively labeled, indicating that the initial DNA damage was localized close to the sensitizer at the nuclear membrane.