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MEASUREMENT OF THE RATE OF UPTAKE AND SUBCELLULAR LOCALIZATION OF PORPHYRINS IN CELLS USING FLUORESCENCE DIGITAL IMAGING MICROSCOPY
Author(s) -
Georgiou G. N.,
Ahmet M. T.,
Houlton A.,
Silver J.,
Cherry R. J.
Publication year - 1994
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1994.tb05058.x
Subject(s) - cytoplasm , subcellular localization , fluorescence , fluorescence microscope , nucleus , chemistry , porphyrin , fluorescence lifetime imaging microscopy , microscopy , biophysics , photochemistry , microbiology and biotechnology , biochemistry , biology , optics , physics
A fluorescence imaging system incorporating a cooled slow‐scan charge‐coupled device camera was used to study the rate of uptake and subcellular localization of porphyrins in living cells. Measurements were carried out on human dermal fibroblasts (D532) using two different porphyrins meso ‐tetra(4‐ N ‐methylpyridyl)porphine (TMPP) and meso ‐tetra(4‐ N ‐hexylpyridyl)porphine (THPP). It was observed that TMPP was rapidly taken up by cells and principally located in the nucleus. The THPP, on the other hand, internalized more slowly and exhibited a particulate distribution in the cytoplasm.