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HEMATOPORPHYRIN DERIVATIVE‐INDUCED PHOTODYNAMIC INHIBITION OF Na + /K + ‐ATPase IN L929 FIBROBLASTS, CHINESE HAMSTER OVARY CELLS AND T24 HUMAN BLADDER TRANSITIONAL CARCINOMA CELLS
Author(s) -
Penning Louis C.,
Tijssen Karmi,
VanSteveninck John,
Dubbelman Tom M. A. R.
Publication year - 1994
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1994.tb05043.x
Subject(s) - chinese hamster ovary cell , hematoporphyrin , photosensitizer , cell culture , microbiology and biotechnology , chemistry , enzyme , hamster , biology , biochemistry , photodynamic therapy , photochemistry , genetics , organic chemistry
The photodynamically induced inhibition of Na + /K + ‐ATPase with hematoporphyrin derivative as photosensitizer was studied in murine L929 fibroblasts, Chinese hamster ovary (CHO) cells and T24 human bladder transitional carcinoma cells. In T24 cells the inhibition of the enzyme activity appeared to be caused by ATP depletion rather than by direct damage from the enzyme itself. In L929 and CHO cells, on the other hand, the inhibition was caused by photodynamic damage from the enzyme molecule. For all three cell lines it was shown that a causal relationship between photodynamically induced reduction in Na + /K + ‐ATPase activity and the loss of clonogenicity is highly unlikely.