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AUTHENTIC in vivo ABSORPTION SPECTRA FOR CHLOROPHYLL IN LEAVES AS DERIVED FROM in situ AND in vitro MEASUREMENTS *
Author(s) -
Richter Thomas,
Fukshansky Leonid
Publication year - 1994
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1994.tb05028.x
Subject(s) - chlorophyll , absorption (acoustics) , in vivo , absorption spectroscopy , spectral line , in situ , chlorophyll a , characterization (materials science) , chemistry , chloroplast , scattering , botany , analytical chemistry (journal) , optics , biology , physics , chromatography , biochemistry , microbiology and biotechnology , organic chemistry , astronomy , gene
On the basis of a comprehensive treatment of light propagation in plant tissue the first authentic in vivo absorption spectrum of chlorophyll in a leaf (of Catalpa bignonioides ) has been elucidated. An experimental‐theoretical procedure is described, which permits derivation of the authentic chlorophyll spectrum by purifying the in vivo absorption spectrum of a leaf from the distortions of optical origin: multiple scattering and distributional error. The procedure involves measurements of internal and external light fluxes, spatial characterization of tissue layers, cells, chloroplasts and grana stacks. Furthermore, one has to determine the local chlorophyll concentrations. The theories of radiation transfer and absorption statistics make up the theoretical basis of the procedure. The derived authentic chlorophyll spectrum differs both from the in vivo spectrum of the leaf and (although to a smaller degree) from the in vitro spectrum of chlorophyll‐protein complexes extracted from the same leaf.